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% P1: The effects of smoking on miRNA expression % P2: The effects of corticosteroids on DNA methylation % P3: To find unique DE genes for Severe COPD in bronchial brushes

% P3: The effects of smoking on RNA Splicing % P4: The effects of corticosteroids on RNA Splicing % P5: Does DNA methylation change RNA Splicing? % P6: Is FEV1 a predictor for fluticasone response? % P7: Which changes in the airway epithelium transcriptome\lead to severe COPD? % P8: Is the persistent inflammation following smoking cessation in COPD driven by specific changes from the initial smoke exposure?